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plasmid pires-hmga1  (Addgene inc)


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    Structured Review

    Addgene inc plasmid pires-hmga1
    Plasmid Pires Hmga1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pires+hmga1/expression+plasmid+pires+hmga1/pm30135148-87-7-9
    Average 90 stars, based on 1 article reviews
    plasmid pires-hmga1 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: MicroRNA-214 suppresses growth, migration and invasion through a novel target, high mobility group AT-hook 1, in human cervical and colorectal cancer cells
    Article Snippet: miRNA-control (no. CN-001000-01-20), miR-214 mimic (no. C-301153-01), siRNA-control (no. D-001220-01-20), siRNA- HMGA1 (no. M-004597-02), antimiR-control (no. IN-002005-01-20 or anti miR-214 (no. IH-301153-02-0005) were obtained from GE Healthcare Dharmacon (Lafayette, CO, USA). .. Transient transfections of the above (with 5 n M of miRNA and antimiR mimics and 50 nM of siRNA mimics) into CaCx and CRC cells were achieved using Lipofectamine RNAiMAX (no. 13778150, Life Technologies) while pcDNA 3.1, pcDNA 3.1- miR-214 , pIRES (vector control), pIRES- HMGA1 (kind gift from Edward Whang, Addgene plasmid no. 13466, Addgene, Cambridge, MA, USA), 3′UTR reporter plasmid constructs were transfected using linear polyethyleneimine (no. 23966-2, MW 25 000, procured from Polysciences, Warrington, PA, USA) at a ratio of 5 : 1 to DNA. .. Combinations of miR-control+pIRES, miR-214 +pIRES, miR-control+pIRES- HMGA1 , miR-214 +pIRES- HMGA1 were transfected using DharmaFECT Duo (no. T-2010-01, GE Healthcare Dharmacon).

    Article Title: miR-214 activates TP53 but suppresses the expression of RELA, CTNNB1, and STAT3 in human cervical and colorectal cancer cells.
    Article Snippet: Cell Biochem Funct.. 2017;1–8.. High Mobility Group AT‐hook 1 (HMGA1) was identified as a target of miR‐214 in human cervical and colorectal cancers (CaCx and CRC) in a previous study.

    Control:

    Article Title: MicroRNA-214 suppresses growth, migration and invasion through a novel target, high mobility group AT-hook 1, in human cervical and colorectal cancer cells
    Article Snippet: miRNA-control (no. CN-001000-01-20), miR-214 mimic (no. C-301153-01), siRNA-control (no. D-001220-01-20), siRNA- HMGA1 (no. M-004597-02), antimiR-control (no. IN-002005-01-20 or anti miR-214 (no. IH-301153-02-0005) were obtained from GE Healthcare Dharmacon (Lafayette, CO, USA). .. Transient transfections of the above (with 5 n M of miRNA and antimiR mimics and 50 nM of siRNA mimics) into CaCx and CRC cells were achieved using Lipofectamine RNAiMAX (no. 13778150, Life Technologies) while pcDNA 3.1, pcDNA 3.1- miR-214 , pIRES (vector control), pIRES- HMGA1 (kind gift from Edward Whang, Addgene plasmid no. 13466, Addgene, Cambridge, MA, USA), 3′UTR reporter plasmid constructs were transfected using linear polyethyleneimine (no. 23966-2, MW 25 000, procured from Polysciences, Warrington, PA, USA) at a ratio of 5 : 1 to DNA. .. Combinations of miR-control+pIRES, miR-214 +pIRES, miR-control+pIRES- HMGA1 , miR-214 +pIRES- HMGA1 were transfected using DharmaFECT Duo (no. T-2010-01, GE Healthcare Dharmacon).

    Article Title: miR-214 activates TP53 but suppresses the expression of RELA, CTNNB1, and STAT3 in human cervical and colorectal cancer cells.
    Article Snippet: Cell Biochem Funct.. 2017;1–8.. High Mobility Group AT‐hook 1 (HMGA1) was identified as a target of miR‐214 in human cervical and colorectal cancers (CaCx and CRC) in a previous study.

    Plasmid Preparation:

    Article Title: MicroRNA-214 suppresses growth, migration and invasion through a novel target, high mobility group AT-hook 1, in human cervical and colorectal cancer cells
    Article Snippet: miRNA-control (no. CN-001000-01-20), miR-214 mimic (no. C-301153-01), siRNA-control (no. D-001220-01-20), siRNA- HMGA1 (no. M-004597-02), antimiR-control (no. IN-002005-01-20 or anti miR-214 (no. IH-301153-02-0005) were obtained from GE Healthcare Dharmacon (Lafayette, CO, USA). .. Transient transfections of the above (with 5 n M of miRNA and antimiR mimics and 50 nM of siRNA mimics) into CaCx and CRC cells were achieved using Lipofectamine RNAiMAX (no. 13778150, Life Technologies) while pcDNA 3.1, pcDNA 3.1- miR-214 , pIRES (vector control), pIRES- HMGA1 (kind gift from Edward Whang, Addgene plasmid no. 13466, Addgene, Cambridge, MA, USA), 3′UTR reporter plasmid constructs were transfected using linear polyethyleneimine (no. 23966-2, MW 25 000, procured from Polysciences, Warrington, PA, USA) at a ratio of 5 : 1 to DNA. .. Combinations of miR-control+pIRES, miR-214 +pIRES, miR-control+pIRES- HMGA1 , miR-214 +pIRES- HMGA1 were transfected using DharmaFECT Duo (no. T-2010-01, GE Healthcare Dharmacon).

    Article Title: miR-214 activates TP53 but suppresses the expression of RELA, CTNNB1, and STAT3 in human cervical and colorectal cancer cells.
    Article Snippet: Cell Biochem Funct.. 2017;1–8.. High Mobility Group AT‐hook 1 (HMGA1) was identified as a target of miR‐214 in human cervical and colorectal cancers (CaCx and CRC) in a previous study.

    Construct:

    Article Title: MicroRNA-214 suppresses growth, migration and invasion through a novel target, high mobility group AT-hook 1, in human cervical and colorectal cancer cells
    Article Snippet: miRNA-control (no. CN-001000-01-20), miR-214 mimic (no. C-301153-01), siRNA-control (no. D-001220-01-20), siRNA- HMGA1 (no. M-004597-02), antimiR-control (no. IN-002005-01-20 or anti miR-214 (no. IH-301153-02-0005) were obtained from GE Healthcare Dharmacon (Lafayette, CO, USA). .. Transient transfections of the above (with 5 n M of miRNA and antimiR mimics and 50 nM of siRNA mimics) into CaCx and CRC cells were achieved using Lipofectamine RNAiMAX (no. 13778150, Life Technologies) while pcDNA 3.1, pcDNA 3.1- miR-214 , pIRES (vector control), pIRES- HMGA1 (kind gift from Edward Whang, Addgene plasmid no. 13466, Addgene, Cambridge, MA, USA), 3′UTR reporter plasmid constructs were transfected using linear polyethyleneimine (no. 23966-2, MW 25 000, procured from Polysciences, Warrington, PA, USA) at a ratio of 5 : 1 to DNA. .. Combinations of miR-control+pIRES, miR-214 +pIRES, miR-control+pIRES- HMGA1 , miR-214 +pIRES- HMGA1 were transfected using DharmaFECT Duo (no. T-2010-01, GE Healthcare Dharmacon).

    Article Title: miR-214 activates TP53 but suppresses the expression of RELA, CTNNB1, and STAT3 in human cervical and colorectal cancer cells.
    Article Snippet: Cell Biochem Funct.. 2017;1–8.. High Mobility Group AT‐hook 1 (HMGA1) was identified as a target of miR‐214 in human cervical and colorectal cancers (CaCx and CRC) in a previous study.

    Luciferase:

    Article Title: miR-214 activates TP53 but suppresses the expression of RELA, CTNNB1, and STAT3 in human cervical and colorectal cancer cells.
    Article Snippet: Cell Biochem Funct.. 2017;1–8.. High Mobility Group AT‐hook 1 (HMGA1) was identified as a target of miR‐214 in human cervical and colorectal cancers (CaCx and CRC) in a previous study.



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    Image Search Results


    HMGA1 is highly expressed in spheroids from A2780 ovarian cancer cells. ( a ) RT-PCR analysis and western blotting analysis of A2780 adherent cells and spheroid cells are shown in the upper panels with HMGA1 probes along with GAPDH probes. Quantification of HMGA expression by western blotting after normalization using GAPDH expression is shown in the lower panel. * P <0.05. ( b , c ) Flow cytometric analysis and sorting of A2780 spheroid cells after incubation with an ALDH substrate is shown. Western blotting analysis of whole spheroid cells, ALDH − cells and ALDH + cells after sorting is shown with the indicated antibodies.

    Journal: Experimental & Molecular Medicine

    Article Title: Crucial role of HMGA1 in the self-renewal and drug resistance of ovarian cancer stem cells

    doi: 10.1038/emm.2016.73

    Figure Lengend Snippet: HMGA1 is highly expressed in spheroids from A2780 ovarian cancer cells. ( a ) RT-PCR analysis and western blotting analysis of A2780 adherent cells and spheroid cells are shown in the upper panels with HMGA1 probes along with GAPDH probes. Quantification of HMGA expression by western blotting after normalization using GAPDH expression is shown in the lower panel. * P <0.05. ( b , c ) Flow cytometric analysis and sorting of A2780 spheroid cells after incubation with an ALDH substrate is shown. Western blotting analysis of whole spheroid cells, ALDH − cells and ALDH + cells after sorting is shown with the indicated antibodies.

    Article Snippet: pIRES-puro3 vectors overexpressing HMGA1 or non-target controls were purchased from Addgene (Plasmid #13466).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Incubation

    Altering HMGA1 expression changes the expression of stemness-related gene expression. ( a ) Western blotting analysis of A2780 spheroid cells with (sh-HMGA1) or without (sh-Control) knockdown of HMGA1 is shown with the indicated probes. ( b ) Western blotting analysis of HMGA1-overexpressing A2780 cells (HMGA1) or control A2780 cells (Control) is shown with the indicated probes. ( c ) The results of flow cytometric analysis of A2780 spheroid cells with HMGA1 knockdown in comparison with control A2780 spheroid cells (upper panel) or HMGA1-overexpressing A2780 adherent cells in comparison with control A2780 adherent cells (lower panel) after incubation with ALDH substrate are shown.

    Journal: Experimental & Molecular Medicine

    Article Title: Crucial role of HMGA1 in the self-renewal and drug resistance of ovarian cancer stem cells

    doi: 10.1038/emm.2016.73

    Figure Lengend Snippet: Altering HMGA1 expression changes the expression of stemness-related gene expression. ( a ) Western blotting analysis of A2780 spheroid cells with (sh-HMGA1) or without (sh-Control) knockdown of HMGA1 is shown with the indicated probes. ( b ) Western blotting analysis of HMGA1-overexpressing A2780 cells (HMGA1) or control A2780 cells (Control) is shown with the indicated probes. ( c ) The results of flow cytometric analysis of A2780 spheroid cells with HMGA1 knockdown in comparison with control A2780 spheroid cells (upper panel) or HMGA1-overexpressing A2780 adherent cells in comparison with control A2780 adherent cells (lower panel) after incubation with ALDH substrate are shown.

    Article Snippet: pIRES-puro3 vectors overexpressing HMGA1 or non-target controls were purchased from Addgene (Plasmid #13466).

    Techniques: Expressing, Gene Expression, Western Blot, Control, Knockdown, Comparison, Incubation

    HMGA1 expression regulates the CSC characteristics of A2780 ovarian cancer cells. ( a ) Time course of the cell numbers counted after plating the same number of cells (1 × 10 4 cells per well). A2780 spheroid cells with HMGA1 knockdown are compared with control A2780 spheroid cells (upper panel), and HMGA1-overexpressing A2780 adherent cells are compared with control A2780 adherent cells (lower panel). * P <0.05 ( b ) Bright-field images of spheroids generated from A2780 spheroid cells with or without HMGA1 knockdown (upper panels) and counts of the spheroid numbers (lower panel) are shown. Scale bar, 100 μm. * P <0.05 ( c ) Bright-field images of spheroids generated from HMGA1-overexpressing A2780 adherent cells in comparison with control A2780 adherent cells (upper panels) and counts of the spheroid numbers (lower panel) are shown. Scale bar, 100 μm. * P <0.05.

    Journal: Experimental & Molecular Medicine

    Article Title: Crucial role of HMGA1 in the self-renewal and drug resistance of ovarian cancer stem cells

    doi: 10.1038/emm.2016.73

    Figure Lengend Snippet: HMGA1 expression regulates the CSC characteristics of A2780 ovarian cancer cells. ( a ) Time course of the cell numbers counted after plating the same number of cells (1 × 10 4 cells per well). A2780 spheroid cells with HMGA1 knockdown are compared with control A2780 spheroid cells (upper panel), and HMGA1-overexpressing A2780 adherent cells are compared with control A2780 adherent cells (lower panel). * P <0.05 ( b ) Bright-field images of spheroids generated from A2780 spheroid cells with or without HMGA1 knockdown (upper panels) and counts of the spheroid numbers (lower panel) are shown. Scale bar, 100 μm. * P <0.05 ( c ) Bright-field images of spheroids generated from HMGA1-overexpressing A2780 adherent cells in comparison with control A2780 adherent cells (upper panels) and counts of the spheroid numbers (lower panel) are shown. Scale bar, 100 μm. * P <0.05.

    Article Snippet: pIRES-puro3 vectors overexpressing HMGA1 or non-target controls were purchased from Addgene (Plasmid #13466).

    Techniques: Expressing, Knockdown, Control, Generated, Comparison

    HMGA1 regulates ABCG2 promoter activity and drug resistance. ( a ) Schematic representations of the −1285 and −628 5′ upstream regions of the ABCG2 promoter are shown. HMGA1-binding sites are marked by boxes. ( b ) The results of a transcriptional activity assay after co-transfecting an ABCG2 promoter construct (−1285 or −628) with an HMGA1-overexpression construct into A2780 cells are shown (upper panel). * P <0.05. The results of western blotting analysis are shown in the lower panel. ( c ) The results of a transcriptional activity assay after co-transfecting an ABCG2 promoter construct (−1285, ΔHMGA1-1, ΔHMGA1-2 or ΔHMGA1-3) with an HMGA1-overexpression construct into A2780 cells are shown (upper panel). * P <0.05. The results of western blotting analysis are shown in the lower panel. ( d ) The results from a time course MTT assay with A2780 spheroid cells with HMGA1 knockdown in comparison with control A2780 spheroid cells (left panel), or with HMGA1-overexpressing A280 cells in comparison with control A2780 adherent cells (right panel) in the presence of paclitaxel (0.1 μ M ), are shown. * P <0.05. ( e ) The results from a time course MTT assay with A2780 spheroid cells with HMGA1 knockdown in comparison with control A2780 spheroid cells (left panel), or with HMGA1-overexpressing A280 adherent cells in comparison with control A2780 adherent cells (right panel) in the presence of doxorubicin (1 μ M ), are shown. * P <0.05.

    Journal: Experimental & Molecular Medicine

    Article Title: Crucial role of HMGA1 in the self-renewal and drug resistance of ovarian cancer stem cells

    doi: 10.1038/emm.2016.73

    Figure Lengend Snippet: HMGA1 regulates ABCG2 promoter activity and drug resistance. ( a ) Schematic representations of the −1285 and −628 5′ upstream regions of the ABCG2 promoter are shown. HMGA1-binding sites are marked by boxes. ( b ) The results of a transcriptional activity assay after co-transfecting an ABCG2 promoter construct (−1285 or −628) with an HMGA1-overexpression construct into A2780 cells are shown (upper panel). * P <0.05. The results of western blotting analysis are shown in the lower panel. ( c ) The results of a transcriptional activity assay after co-transfecting an ABCG2 promoter construct (−1285, ΔHMGA1-1, ΔHMGA1-2 or ΔHMGA1-3) with an HMGA1-overexpression construct into A2780 cells are shown (upper panel). * P <0.05. The results of western blotting analysis are shown in the lower panel. ( d ) The results from a time course MTT assay with A2780 spheroid cells with HMGA1 knockdown in comparison with control A2780 spheroid cells (left panel), or with HMGA1-overexpressing A280 cells in comparison with control A2780 adherent cells (right panel) in the presence of paclitaxel (0.1 μ M ), are shown. * P <0.05. ( e ) The results from a time course MTT assay with A2780 spheroid cells with HMGA1 knockdown in comparison with control A2780 spheroid cells (left panel), or with HMGA1-overexpressing A280 adherent cells in comparison with control A2780 adherent cells (right panel) in the presence of doxorubicin (1 μ M ), are shown. * P <0.05.

    Article Snippet: pIRES-puro3 vectors overexpressing HMGA1 or non-target controls were purchased from Addgene (Plasmid #13466).

    Techniques: Activity Assay, Binding Assay, Construct, Over Expression, Western Blot, MTT Assay, Knockdown, Comparison, Control

    HMGA1 overexpression enhances the drug resistance of ovarian cancer cells in a xenograft tumor model. ( a ) Tumor size was measured on the indicated day after the subcutaneous injection of HMGA1-overexpressing A2780 or control A2780 cells into nude mice (1 × 10 5 per injection) with HBSS or paclitaxel treatment ( n =6). Paclitaxel was injected at each data point. * P <0.05. ( b ) Immunohistochemistry results of tumors harvested on day 45. DAPI and the indicated antibodies are shown. Scale bar, 50 μm.

    Journal: Experimental & Molecular Medicine

    Article Title: Crucial role of HMGA1 in the self-renewal and drug resistance of ovarian cancer stem cells

    doi: 10.1038/emm.2016.73

    Figure Lengend Snippet: HMGA1 overexpression enhances the drug resistance of ovarian cancer cells in a xenograft tumor model. ( a ) Tumor size was measured on the indicated day after the subcutaneous injection of HMGA1-overexpressing A2780 or control A2780 cells into nude mice (1 × 10 5 per injection) with HBSS or paclitaxel treatment ( n =6). Paclitaxel was injected at each data point. * P <0.05. ( b ) Immunohistochemistry results of tumors harvested on day 45. DAPI and the indicated antibodies are shown. Scale bar, 50 μm.

    Article Snippet: pIRES-puro3 vectors overexpressing HMGA1 or non-target controls were purchased from Addgene (Plasmid #13466).

    Techniques: Over Expression, Injection, Control, Immunohistochemistry